Abstract A novel method to create nanoparticles from freeze- and air-dried mangosteen was developed to study the effects of these particles on the growth and protein formation of various gram-positive bacteria that act as foodborne pathogens. This new method produces freeze- and air-dried mangosteen peel nanoparticles that are prepared by a process based on a wet-milling technique, and these particles were tested on various gram-positive pathogenic bacteria. Our results indicated that the nanoparticles derived from freeze dried mangosteen contained higher antioxidant activity than nanoparticles derived from air-dried peels. The total phenol content in the freeze-dried nanoparticle extract was 1112.646 ± 1.842 (mg gallic acid /g sample), whereas that in the air-dried extract was 479.744 ± 2.564 (mg gallic acid/g sample). The total flavonoids in the mangosteen freeze-dried nanoparticle extract were 14.154 ± 0.119 (mg catechin/g sample), whereas levels in the air-dried extract were 4.711 ± 0.207 (mg catechin/g sample). The levels of 2,2-diphenyl-1-picrylhydrazyl (DPPH) were 95.707 ± 0.070 and 94.303 ± 0.074% for freeze- and air-dried mangosteen nanoparticle extracts, respectively. Similar levels were obtained for 2,4,6-tri (2-pyridyl)-s-triazine (ABTS), and these were 42.753 ± 0.200 and 16.069 ± 0.424 (g trolox/g sample) for freeze- and air-dried mangosteen nanoparticle extracts, respectively. Levels of ferric reducing antioxidant power (FRAP) were 17.806 ± 0.056 and 6.696 ± 0.085 (g trolox/g sample) for freeze- and air-dried mangosteen nanoparticle extracts, respectively. Whole protein bands from various bacteria disappeared on SDS-polyacrylamide gels when bacteria were cultured in medium containing both freeze- and air-dried mangosteen nanoparticles.

Abstract Breast cancer is the greatest common malignancy in females. This syndrome represents a serious public health problem that needs additional study to define its prediction and specific treatment. MCF-7 is a usually used breast cancer cell line that has been spread for many years by multiple groups. It shows to be a suitable model cell line for breast cancer studies worldwide, including those concerning anticancer. Anticancer and anti-inflammatory influences are important to treat the threats of chronic inflammation related with chronic diseases. Natural products have made and are remaining to make vital influences to the search for new anticancer agents. The use of natural foodstuffs in relationship with green nanoparticles has drawn attention because of its easy and eco-friendly protocol. Therefore, we aimed to investigate the antiproliferative activity of silver nanoparticles biosynthesized by mangosteen peel extract (MPE) on human breast cancer cell line (MCF-7) using [3-(4, 5-dimethylthiazolyl)-2, 5-diphenyl-tetrazolium bromide (MTT) method. In the present study serial concentrations (5, 10, 15, 50, 70, µg/mL) of cells biosynthesized silver nanoparticles by (MPE) was tested against MCF-7 cell lines showed a cytotoxic effects, morphological degeneration, damaging and has more effectiveness against breast cancer cells. The results showed a substantial a high reducing power, a sensible antiproliferative role and antioxidant activity. The results showed that silver nanoparticles biosynthesized by mangosteen peel extract (MPE) can be regarded as a suitable candidate for designing anticancer pharmaceutical preparations.